概要

顔写真 Name: Ken NISHIMURA
Affiliation: Faculty of Medicine
Specialty: Major of Medical Science
Field of Research: Stem cell engineering, Molecular cell biology
Position: Assistant Professor
Degree: Ph.D. (Medicine)
Degree earning University : Graduate School of Medicine, Osaka University (March 2003)
Starting Date: August 1, 2012
Mentor: Professor Koji Hisatake
Laboratory: http://www.md.tsukuba.ac.jp/
          basic-med/biochem/gene/eng/nishimura_top.html

Analysis of transcriptional regulation of cell reprogramming and development of practical systems of cell differentiation.

 Induced pluripotent stem (iPS) cells can be produced by introducing several genes into mature somatic cells. These cells are expected to be useful for many applications including regenerative medicine. However, the mechanisms of the cell reprogramming remain largely unknown. Their understanding is expected to not only enable more efficient production of iPS cells but also give us important information about the maintenance of the undifferentiated status and the orderly differentiation of these cells.
 We developed “Persistent RNA vector” which is capable of sustained expression of multiple transgenes without chromosomal integration. This vector can produce transgene-free iPS cells more efficiently than conventional methods.
 Using this method of efficient iPS cell production, we try to identify transcription factors relating to each step of cell reprogramming, and investigate molecular mechanism of cell reprogramming by focusing on functions of these transcription factors. And these analyses will make us enable to establish methods of more efficient production of safe iPS cells. Moreover, we also apply persistent RNA vector to obtain safe differentiated tissue cells efficiently. Taken together, these researches will contribute great progress of medical application of regenerative medicine, from iPS cell production to preparation of differentiated tissue.

 

 

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